Anti-COBRA1 (NELF B) Antibody

Product Name

Anti-COBRA1 (NELF B) Antibody

View all NELFB Antibodies

SKU/Catalog Number

A12025

Size

100 µl

Form

Liquid

Description

Boster Bio Anti-COBRA1 (NELF B) Antibody (Catalog # A12025). Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) upon arrival and stored long term at -20° C or -80° C, while keeping a working aliquot stored at 4° C for short term. Avoid freeze/thaw cycles. Stable for at least 1 year. After date of receipt, stable for at least 1 year at -20°C.

Cite This Product

Anti-COBRA1 (NELF B) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A12025)

Host

Mouse

Contents

PBS + 0.05% NaN3

Clonality

Monoclonal

Clone Number

F7E4

Isotype

IgG1

Immunogen

Recombinant full length human COBRA1 purified from E. coli. Immunogen species is Human.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A12025 is reactive to NELFB in Human, Mouse, Rat

Applications

A12025 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

65.697kDa

Background of NELFB

COBRA1 (cofactor of BRCA1) also known as NELF-B, is an essential component of the negative elongation factor (NELF) complex. COBRA1 has been shown to directly bind estrogen receptor alpha (ERα) and repress ERα-mediated transcription (Aiyar SE et al., 2004). COBRA1 has also been demonstrated to interact with c-Jun and c-Fos suggesting a role in modulation of the AP-1 pathway and therefore may be important in cell proliferation, differentiation, apoptosis and oncogenesis (Zhong H et al., 2004)

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB: 1:1000

Validation Images & Assay Conditions

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Western blot of neonatal rat brain lysate showing specific immunolabeling of the ~62 kDa COBRA1 protein.

Gene/Protein Information For NELFB (Source: Uniprot.org, NCBI)

Alternative Names

Negative elongation factor B NELFB COBRA1, NELF-B negative elongation factor complex member B negative elongation factor B|cofactor of BRCA1|negative elongation factor protein B

For more info on NELFB, check out the NELFB Infographic

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In this infographic, you will see the following information for NELFB: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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